EXPRESSION OF GENETIC MARKERS OF ENDOTHELIAL PATHOLOGY IN PREGNANT WOMEN

Problem declaration. Pregnancy considers to be reputable threat aspect for venous system conditions. The compound of cardio-vascular changes throughout pregnancy provides considerable load on veins, which increases with gestational age and might be accompanied by venous endothelial damage. This in turn can cause the advancement of perinatal issues associated with endothelial dysfunction.The detection of biochemical markers of venous endothelial disfunction might help early medical diagnosis of pregnancy complications and expand the possibilities of preventive therapy.The objective: to study the features of expression of markers of endothelial vein dysfunction in women depending on pregnancy outcomes.Methods: There were 100 pregnant women in whom the expression of adhesion molecules ICAM-1, VCAM-1 and hypoxia gene HIF 1 were identified in the dynamics of pregnancy. Analysis of gene expression was performed by real-time PCR utilizing business probes and primers (Applied Biosystems, Hs00234077 _ m1) on a CFX96 thermocycler (BioRad, U.S.A.). The recommendation gene used gene ACTB (Hs01060665 _ g1). At the end of pregnancy, women who brought to life children with pathology of the early neonatal period were determined. The analysis of ICAM-1, VCAM-1 and hypoxia gene HIF 1 was carried out depending on pregnancy results for the fetus.Results. In women who brought to life children with issues of the early neonatal duration the expression of the cell adhesion particle ICAM1 is 6.1 times higher than in ladies who delivered without complications. Such dynamics is observed in the expression of Hif1a gene u2013 its expression is higher by 9.7 times in women who gave birth to children with pathology. VCAM 1 gene activity was not detected in any research study group.Conclusion. The study of the expression of adhesion particles ICAM-1, VCAM-1 and hypoxia gene HIF 1 showed promising worth for predicting problems of the neonatal duration.

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